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KMID : 0358219740010010049
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Korean Journal of Fertility and Sterility
1974 Volume.1 No. 1 p.49 ~ p.54
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Effect of Progesterone on the Germinal Vesicle Break-down of Mouse Oocytes in Vitro
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趙完圭/Cho, Wan Kyoo
權赫邦/鄭淳五/Kwon, Hyuk Bang/Chung, Soon O
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Abstract
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In the present studies, effect of progesterone on the germinal vesicle break-down of the mouse oocytes cultured in the micro tube was investigated. The results obtained are as follows: As dose of progesterone in the medium rose, accordingly the break-down of the germinal vesicle was suppressed. It was found that ED50 was 15.7 pg1ml, and ED90 60.7.mg/ml of progesterone. The dose suppressing the oocyte maturation was apparently higher than that on the rabbit or on. the mouse embryonal development. The inhibiting effect of progesterone on the GVBD was reversible. The germinal vesicle of the oocytes were broken down immediately in the medium upon removal of the hormone. Progesterone stops meiosis at any stage upon administration, while dbe AMP or theophylline supresses only the break-down of the nuclear membrane. Recovering of the meiotic division of the oocytes once exposed to progesterone was delayed a little. The inhibiting action of progesterone was not altered by adding more pyruvate or in the presence of higher concentration of the mineral ions in the culture medium.
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